FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752647
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAGAGTATCTCGTATGCCGT31610.18035577044322101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9100.041.9709931
TCTCGCT10350.036.902042
TCGCTCT9400.036.7668384
TCTGTCG7450.033.8234188
CTCGCTC10500.032.2569543
CGCTCTG10050.031.5231445
GTCTTAC5900.030.5353431
GTCTTGC14300.029.2285181
TATCGCA1050.00797386727.4292284
TACACCG1358.4174564E-426.6673055
GTATTGC3255.4569682E-1126.6080281
CTGTCGC11000.026.1802049
GTCTCAC16850.025.2328831
GTACTAA2351.4032976E-624.5322251
GTGTTAA5600.024.4500851
TCTGTCA18100.024.2636998
GTCTAAA5350.024.2456341
TTGCTCT18450.024.19574
GTTTTAA6300.024.0211371
GTATAAT2401.6855338E-624.0211371
Produced by FastQC (version 0.11.5)