FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2301181
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8950.041.0546881
TCTGTCG10000.038.8850638
TCGCTCT11100.033.728264
ACCGTGT4850.029.6945888
GTCTTGC17800.029.5473461
CTCGCTC11500.028.1726863
TTCACCG5150.027.9599465
CTGTCGC13500.027.2035439
CGCTCTG11600.026.6884925
TCTCGCT12200.025.9711592
GTTTCAC28250.024.2113323
CCGTGTT5250.023.3173249
GTCTCAC24050.022.7674371
TTGCTCT24100.022.7044034
GTATTAG5950.021.7957171
GGGTTTC31500.021.7284451
GTCTTAC6650.021.6682571
CTCGATC8150.021.2052158
GTACAGG9200.021.1442151
TCACTCT27950.020.607334
Produced by FastQC (version 0.11.5)