FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163438
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGCATAATCTCGTATGCCGT66280.569690864489556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG4650.044.9030238
GTCTCGC5200.037.3876571
TACCGTT800.00210672136.0013857
GGTACGT1102.5361893E-432.729941
GTACTAT2009.569703E-932.402641
TCGCTCT4900.032.3277744
GTCTTGC8950.032.1813931
CGTCAGA2051.2174496E-831.6123311
TCTCGCT5950.029.0431352
CTGTCGC7250.028.7998719
CTCGCTC6250.026.497023
GTACAAC1959.23339E-625.848261
GTGCCGA1701.0804964E-425.4138341
TAACACG2301.1715711E-625.0444434
GTTTAAA4900.024.9816281
GTGTAAC2651.4708348E-724.4548241
GTCCGGG3402.7121132E-923.2960151
GGTATAC2502.4193396E-623.0418781
GTGTTAT4104.1836756E-1122.831131
GTCTCAC10500.022.6304131
Produced by FastQC (version 0.11.5)