FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348292

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348292
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163438
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGA750.00153410438.4035452
TCTGTCG4150.034.7019968
GTCTTAC2750.034.0643961
TAACGAC850.00283730533.881113
GCGTTAC900.003738434532.0263561
GTCTCGC4550.031.6744161
GTCTACG1153.2775293E-431.3301281
GTATAAC1153.2775293E-431.3301281
TCGCTCT5550.029.8367424
TCTCGCT5050.028.5174832
TTACTCT5150.027.960144
CGCTCTG6050.027.3708975
CTCGCTC5800.027.3093413
TAACACT5400.026.6656884
GTATTAC2551.00659236E-725.4326921
GTCTTGC8700.024.8480321
GTACTAA1751.3154867E-424.687996
GGCTAGA5850.024.6356541
GTGCTAG4950.023.2918931
GTACTAC1550.001873964823.2449341
Produced by FastQC (version 0.11.5)