FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2229009
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9500.040.1997831
TCGCTCT9550.039.9570164
TCTGTCG9250.038.144638
GTCTTGC19950.033.95131
TCTCGCT11500.032.5636632
CTCGCTC11700.031.9991233
GGTATAC4350.031.472831
CGCTCTG12050.031.0696895
CTGTCGC11950.029.5261769
GTGTAAA12250.027.6460591
TTGCTCT27700.027.291674
GTTTCAC29850.027.2555353
GGGTTTC35550.025.74161
TACACCG1400.001041209625.713585
TACTCCG2001.1252585E-525.199315
CTTGCTC28800.024.7493213
GTATTAT6600.024.018741
TGTACGG1500.001554327924.0052662
CTGTTGC24200.023.8042039
GTGTTAC8800.023.7457961
Produced by FastQC (version 0.11.5)