FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348297

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348297
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530398
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10150.056.7482458
GTCTCGC11250.044.8048971
GTCGTAC1202.0835614E-742.0045931
TCGCTCT11700.037.53964
CTGTCGC15700.035.770379
TCTCGCT13750.034.5610472
GTCTTGC21750.034.4313551
CTCGCTC13750.031.9427853
CGCTCTG13700.030.4826765
ACCGTGT8600.030.139268
GGGTTTC38850.029.0997091
TTCACCG8250.027.0553675
GTTTCAC35700.026.8243453
GTCTCAC26600.025.9878041
CCGTGTT9750.025.8459159
GTATAGA7000.025.7170981
GTATTAC5650.025.4895111
GTATTAG8100.024.8916111
TTGCTCT29100.024.743024
GTGTAGA12600.024.5741181
Produced by FastQC (version 0.11.5)