FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168703
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9400.054.3822948
GTCTCGC9500.048.5124781
TCGCTCT10350.040.3519674
CTGTCGC14200.039.041769
CGCTATA750.001534318238.4039422
TCTCGCT11400.036.0036962
GTCTTGC20900.034.4548871
CTCGCTC12250.032.9176673
CGCTCTG12250.031.7420355
GTCTCAC23600.028.6822321
GTGTTAC6100.027.1515791
TAATACT6400.027.002774
TTCACCG5900.026.8502125
GTTAGCC11600.026.6923943
GTACTAA4050.026.6706351
GTTTCAC28800.026.502723
GTCTTAC6800.026.4745251
GTATTAA5500.026.1857151
GGGTTTC33150.025.6327721
CTGTTGC23150.025.5029169
Produced by FastQC (version 0.11.5)