FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348307

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348307
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861809
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7900.053.7703178
CTGTCGC9750.046.521499
GTCTCGC8500.044.9046441
GTCTTGC18300.038.1728821
TCGCTCT10200.036.7125174
TCTCGCT10400.035.314072
CGCTCTG10450.033.766875
GTCTTAC7350.032.334111
CTCGCTC11700.031.390293
GGTATAC3300.030.5526181
GTTTCAC26650.029.4537283
TTGCTCT23100.028.680514
GGGTTTC31100.028.4825611
GTGTTAC7350.028.4148251
TAATACT5600.028.2908294
TACACTA3850.028.0570185
GTATTAA5500.027.4973561
TACGGTT1050.00796789427.433534
GTGTTAA8450.027.2726671
CCGTGTT7200.026.9990799
Produced by FastQC (version 0.11.5)