FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318617
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9700.045.2861988
GTCTCGC8950.043.46951
GTCTTGC20200.034.953331
TCGCTCT9600.032.2486344
TCTCGCT11000.032.078982
CTGTCGC14300.031.2221939
GTCTTAC8200.029.8730091
CTCGCTC10950.028.930283
CGCTCTG10500.028.1130945
GGGTAAC7900.024.6235451
GTGTAAC6850.024.1908511
GTCCAGA15600.024.0155561
GTCTCAC25850.023.9691051
GTATAGA7650.023.5446621
GTATTAA6450.023.4570521
GTGTTAG15250.023.149421
CTCGATC8250.022.6948418
ACTGTCG2552.8731392E-622.592158
GTACAGT11300.022.315341
GAGTGCA55850.022.1786
Produced by FastQC (version 0.11.5)