FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2305844
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC19950.042.6147881
TCTGTCG9850.040.2044838
GTCTCGC10250.038.659841
TCGCTCT10500.033.600474
GTATTAG6700.031.1849061
CTCGCTC11650.030.901723
CTGTCGC13750.030.8956479
CGCTCTG11350.029.1810255
TTGCTCT28300.029.003944
TCTCGCT13200.028.9094962
TCTGTCA28050.027.7229168
GTCTCAC26450.027.2392751
GTGTTAC8150.026.5206931
GTATAGA6850.026.2948471
GGTATAC2851.0591975E-825.2799591
CTGTCAC33250.024.9031459
GTATTAA5500.024.8892691
TCTTGCT32000.024.5253432
CTTGCTC29700.024.4851913
GTTTCAC31250.023.7315313
Produced by FastQC (version 0.11.5)