FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281535
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10000.043.1986358
CTGTCGC12000.037.79889
GTCTCGC10100.037.0868261
GTCTTGC20450.035.9289551
TCGCTCT11300.035.0592774
GTGTTAG16950.030.1735461
TCTCGCT12300.029.8664592
CTCGCTC11900.029.6597733
GTATAAC3850.028.0652071
GGTATAC4150.027.7721581
TACCCGT1306.754544E-427.6914315
CGCTCTG12100.026.7760125
GTCTCAC25250.026.2460631
TTGCTCT28450.025.3184074
TATACTG6050.024.9909445
TCTGTCA26100.024.8268018
GGAGTGC54200.023.3793335
CTTGCTC29100.023.2677043
CGTTTCG1550.001883858323.2250739
GTCTAGA10100.022.8226621
Produced by FastQC (version 0.11.5)