FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348320

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348320
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2281535
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC18650.038.992661
GTCTCGC10300.037.0492171
GTATTAA5450.035.6703721
TCTGTCG9800.035.2628568
TTGCTCT24800.028.452134
TCGCTCT10900.028.4041844
GTATTAG7900.027.3422681
GTATTAT5800.027.3108391
ATACCCG1856.1412284E-627.2437364
CTCGCTC12300.026.3419423
TCTCGCT13250.026.0834922
GTATAAG6000.025.2004591
CTGTCGC14000.025.2004579
CTTGCTC24500.024.6861613
GTGCTAC6800.024.3533821
TCTGTCA26650.024.0433568
CGCTCTG12650.023.9050465
GTCTAGC8450.023.8584211
GTAATAC5150.023.7674223
TTACTCT12500.023.0404174
Produced by FastQC (version 0.11.5)