FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878312
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9500.049.262351
TCGCTCT9350.043.1222844
GTCTTGC15600.039.2301251
TCTGTCG9300.037.9328428
TCTCGCT11700.036.9224662
CTCGCTC11450.035.8422053
CGCTCTG12050.034.057535
CTGTCGC13500.029.3328519
CTGTTGC19200.028.499539
TCCGTAC1358.419668E-426.6662273
TTGCTCT23200.025.7581984
TTACACT7050.025.5314944
CTTGCTC22300.025.5063083
GTGTTAG10300.025.1646351
GTCTCAC21800.025.1005041
CTCGATC7500.024.9582618
TCTGTTG20100.024.7146978
GTATTGC4750.024.2522341
GTGTTAC7150.024.1674351
GTGTAAA9900.023.9996051
Produced by FastQC (version 0.11.5)