FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907755
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6900.049.0408368
GTCTCGC8650.039.972561
GTCTTGC16250.036.3494031
CTGTCGC9450.034.2838679
TCGCTCT9300.033.3024944
GTACTAC2603.6379788E-1233.2464071
GTCTTAC6650.029.2468381
GTCTCAC22450.029.1985871
TCTCGCT10200.028.9517572
GTGTTAC7450.028.0400331
GGGTTTC27750.027.7752271
GTATCGC1306.734339E-427.705341
GTCCTAC4700.027.5874421
CTCGCTC10500.026.7526363
CGCTCTG9700.026.720215
GTAACGC1100.009987771526.1913913
GTTTCAC25100.025.8261723
GGTATAC3751.2732926E-1124.9717451
GTGCTAC5900.024.4182621
TTGCTCT22850.023.956244
Produced by FastQC (version 0.11.5)