FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1907755
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9550.045.990981
TCTGTCG8450.045.1588178
TCGCTCT9700.043.05294
CGCTCTG10850.037.162465
TCTCGCT11850.037.0644572
GTCTTGC15200.036.9485321
CTCGCTC11250.035.841133
GTATTAG5700.031.5799431
CTGTCGC14000.029.3136719
GTGTTAG12900.027.9078561
GGGTTTC28350.027.1754591
GTTTCAC27300.027.165693
GTCTCAC20950.027.1512131
GCGTAAA1607.1593E-527.0008491
TTGCTCT21700.026.5446154
TAATACT5350.025.5708984
GTATAAC2851.0661097E-825.2639541
GGAGTGC46100.024.989945
GAGTGCA49300.024.9744176
GTATATA7500.024.9607871
Produced by FastQC (version 0.11.5)