FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1893571
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6450.049.1151478
GTCTCGC7250.038.7454831
GTCTTGC17950.035.7124821
CTGTCGC10450.033.7599879
GTCTTAC6150.032.792721
TCGCTCT9100.032.449954
TCTCGCT9500.030.32552
CGCTCTG9250.029.5776965
GGTATAC4150.027.7693941
TTGCTCT22600.027.088324
CTCGCTC9850.027.0543463
GTGTTAG12400.026.1387831
GTATTAC4450.025.8972991
GTTACCG1400.001041125525.713698
CCGCCGT1400.001041125525.713699
CTTGCTC21400.025.5782813
TATACTT5100.025.4111795
TTATACT4900.024.9875934
CTTACGG1450.00127496524.8355393
GTATTAG5900.024.4158881
Produced by FastQC (version 0.11.5)