FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1893571
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9350.043.8907138
GTCTCGC7650.037.6470451
CTCGCTC8250.035.7818073
TCGCTCT8500.034.7293974
GTATTAC3650.033.5342371
CTGTCGC12550.030.9800669
GAACCGT1402.880993E-530.857136
GGTCTAC3050.030.6885151
TCTCGCT9350.030.0320742
GTCTTGC15350.030.0195351
GTATTAG7000.029.8285621
GTCTCAC19100.028.2722421
AGTCCGT1804.9630125E-627.999996
CGCTCTG10550.027.9810335
GTATTAT6950.027.9712121
GTACGAA1050.0079749727.4285641
GTACTAA4200.025.714281
GTCTAGA8050.025.043471
CCGTGTT5500.024.8727199
GTACTAG4101.8189894E-1224.5853561
Produced by FastQC (version 0.11.5)