FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074804
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8600.043.5337038
GTCTTGC16900.037.5118061
TCGCTCT9800.037.4855354
GTCTCGC9050.036.616871
TACACCG1551.5477563E-632.5152475
GTATTAT5550.032.450321
CTGTCGC11550.031.1679869
GTATAAC3250.031.0324921
CTCGCTC11500.030.6914833
TCTCGCT11350.030.4624612
CGCTCTG10650.029.0696335
TTGCTCT24350.027.5108264
GTGTAAC7150.027.2038081
GTGTTAA8700.026.4973661
AATACCG1658.8303816E-526.1811085
GCGTTAA1400.001037674625.728471
GTATATA8200.025.4774591
GTCTCAC24250.024.9539621
GTGTTAG13150.024.6523721
GTGTTAC7700.024.3250981
Produced by FastQC (version 0.11.5)