FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074804
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8900.034.7833068
GTCTCGC8400.029.1408651
GTCTTAC7500.027.8380991
TCGCTCT9350.027.7200344
GTATAAT6950.026.9334131
GTTTCAC29650.025.2529143
GGGTTTC32050.025.158971
TATACTG6600.025.088595
GGAGTGC44750.024.7753645
GGTATAC4400.024.543781
GTCCTAT5300.024.451161
GAGTGCA48750.024.21936
CGCTCTG9900.023.9977845
CTCGATC7550.023.8388588
GTGTTAC8450.023.0043451
GTCTTGC16350.022.8975181
CTGTCGC13600.022.76379
GTACTCG1902.3006003E-422.7352891
GTGTAAT7600.022.7352891
TATAATA5850.022.1523322
Produced by FastQC (version 0.11.5)