FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348337

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348337
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9319511
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG43700.027.018388
GTATTAG18550.026.4283751
GTGTTAG58450.024.2989311
GTCTCGC51450.022.7004831
GTATTAT18450.022.2732681
GTCTTGC99750.022.1163771
GTGTTAC35200.021.9152571
GTATAAC14800.021.4336641
GTTAGCC58050.020.2234273
TCGCTCT63400.020.2132874
GTATATA19600.019.8629231
GTATAAT16550.019.6028841
GTATTAC19350.019.3743821
GTATTAA17350.019.1145381
TGTTAGC62650.019.0841452
GTCTTAC35950.019.0515441
GTGTAAT33100.018.0782151
GTGTATA30350.018.0534571
CTGTCGC66050.017.98499
GTATAGA21450.017.8136941
Produced by FastQC (version 0.11.5)