FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348338

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348338
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9319511
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19350.025.751221
GTATTAA17550.023.3819521
GTCTCGC49500.020.7249131
GTGTAAC24950.020.558781
GTCTTGC93800.020.2333051
GTGTTAG57800.020.0308381
TCTGTCG48250.019.2470958
GTTAGCC58000.019.1152293
GGTATAC18150.018.9754091
GTATAGC16100.018.6606981
GTGTAAT29650.018.535591
TGTTAGC60550.018.087172
GTCTTAC34950.018.0310571
GTATATA19700.017.8543831
GTATAGG21350.017.8474161
GTATAAG19550.017.2417341
GTGTTAC33500.017.0615981
TAATACT19700.016.8104134
TCGCTCT61400.016.7669644
GTATAAT20550.016.7593021
Produced by FastQC (version 0.11.5)