FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348341

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348341
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972537
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8500.044.9195751
TCTGTCG7050.042.8929258
TCGCTCT8850.041.5108454
CTGTCGC8100.039.1104779
TCTCGCT9950.035.47382
CTCGCTC9300.034.0803763
CGCTCTG10900.031.0453665
GTCTTGC18150.030.9596981
GTACTAG3700.029.2057441
GTATATA7200.025.014181
GTGTTAC7500.024.9741571
GTATTAG6650.024.9163781
GTATAAG5600.024.4424271
GTACTAT3851.8189894E-1124.3254781
GTAATAC4800.024.0111753
TCTGTCA23550.023.8467488
GTGTTAG13900.023.840851
TTCACCG5900.023.1860665
GTGTAAG10000.023.0530681
TTGCTCT22750.022.7974244
Produced by FastQC (version 0.11.5)