FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348342

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348342
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972537
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC7650.040.4688571
GTCTCGC8150.034.4525151
TATGCGT1708.3235136E-833.8809054
GCGTAAC850.002837865833.8809051
TCGCTCT8000.033.2985764
TCTGTCG8900.032.3581628
CGCTCTG8250.028.7987675
CTCGCTC9400.028.339213
GTCTTGC17850.027.0240541
GTCTCAC21600.026.9988461
GTATGCG1607.163042E-526.9988443
GTATTAG6000.025.1989231
TCTCGCT9950.024.6019632
GTTCTAC5150.023.7659741
CTGTCGC12550.023.5209069
TGCTACG1851.9200446E-423.3503512
GTGTTAG11300.022.937071
GTGTAAA10300.022.3679751
TTATACT6550.021.9837934
TACACCG1650.002709070521.817255
Produced by FastQC (version 0.11.5)