FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348349

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348349
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092925
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8100.039.998298
GTCTCGC9000.035.2128031
GTCTTGC19400.033.414211
TCGCTCT9450.032.7706874
CGTTTCG1153.2951054E-431.3030139
CTGTCGC10350.030.6073919
GTATTAA6600.028.373951
TCTCGCT10800.028.0075042
GTGTATA9700.026.7313691
CGCTCTG11650.026.5739735
TAACACT9250.025.6933694
CTCGCTC11800.025.6339873
GTCTCAC21800.025.4404411
GTCTAGG10300.025.1741981
GTACTAA4300.025.1254121
GTGTTAT7500.024.9690761
GTGTAAA10700.024.906251
GTCTTAG7650.024.4794861
GTCCTAC5650.024.2211951
GTTACCC7250.023.840873
Produced by FastQC (version 0.11.5)