FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348355

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348355
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270063
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8850.043.9313748
GTCTCGC8550.042.1248861
TCGCTCT10200.036.0143974
CTGTCGC11650.033.9907769
CGCTCTG11100.029.8372785
TCTCGCT11600.029.8050162
GTACTAA3151.8189894E-1229.728131
GTCTTGC16850.029.497421
CTCGCTC11350.027.9230563
GTCTCAC23100.027.4413551
TACCCGT1400.001041162925.7138025
GTATAGA6150.024.5968231
GTCCTAG8550.024.4324321
TTGCTCT25800.024.2887784
GGTTACG1500.001552107324.0111851
GTATTAG6650.023.8306481
GTGTAAA11450.023.2772181
GTGTTAA7900.022.7954271
GTATTAC4205.638867E-1122.2960991
GTATAGT5850.022.164171
Produced by FastQC (version 0.11.5)