FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348356

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348356
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2270063
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.038.9167448
GTCTCGC9800.034.529441
GTCTTGC18250.033.927621
TCGCTCT10300.032.154254
CTCGCTC10100.030.6524283
GTCTCAC21400.028.5971641
TCTCGCT12400.027.8700262
GTCTAGC8350.026.7296351
CGCTCTG11000.026.1809335
GTATTAG4500.025.5991331
TCACTCT26200.024.1823884
TCTGTCA25100.024.094278
CTGTCGC15050.023.918939
GGGTAAA8500.023.7168451
GTACAGA8550.022.7360731
GTGTTAG14300.022.6565761
GTGTAAC4455.456968E-1222.6509191
TTACACT8300.022.5534534
GGAGTGC51000.022.1639565
GTCATAA5900.021.9653591
Produced by FastQC (version 0.11.5)