FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348358

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348358
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9858683
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG21200.024.5294841
GTGTTAG61650.022.8104611
GTCTTGC97850.022.006571
GTATTAT22650.021.9890581
GTATTAA18800.021.8170531
TCTGTCG53900.021.10288
GTGTAAA43450.021.0710491
GTCTCGC53400.020.9853421
GTATAAC13950.020.4765281
GTATATA22600.020.4172861
GTTAGCC62650.019.9945163
TGTTAGC67500.019.3201332
GTATAGA23600.018.9314441
GTACTAT18000.017.9038331
GTGTTAA30000.017.8224541
GTATAAG21750.017.1742121
TCGCTCT67100.017.166444
GTGTAAT33550.017.0281721
GTCTTAC39650.016.809861
GTGTAAC24850.016.8001961
Produced by FastQC (version 0.11.5)