FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348359

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348359
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866084
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11300.044.599088
TCGCTCT12000.043.7988854
GTCTCGC12050.042.170451
GGTATAC5750.035.3498341
TCTCGCT14400.034.999112
CTCGCTC13400.034.3871843
GTCTTGC29850.033.317581
CTGTCGC16450.031.5117769
CGCTCTG14350.029.6020345
GTATTAG9600.027.9787141
GTGCTAC10650.027.946761
TAATACT7850.027.5152234
GTATAAT7600.026.7449421
TTCACCG6900.026.0862945
GTATAGG7800.026.0591741
GTGTTAG18950.026.049351
GTATTAT8400.025.926221
GTGTTAC10550.025.4593011
GGGTTTC44500.025.4484791
GTTTCAC40600.025.3589613
Produced by FastQC (version 0.11.5)