FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866084
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12950.042.1917848
GTCTCGC13000.042.0713421
TCGCTCT12600.038.2285924
GTCTTGC24650.034.7413061
CTGTCGC16550.033.4485139
TCTCGCT16350.031.2275812
CGCTCTG15400.030.3442695
CTCGCTC15700.029.7644463
TAGTACT5850.029.494394
GTCTCAC31600.028.0113561
GTGTTAG17800.026.6833341
TAATCCG1358.493682E-426.626885
GTATTAG9550.025.620741
GTGTAAA15650.024.371241
GGGTTTC44100.024.3144151
GGTATAC6550.024.1711621
ATAGTAC7150.024.1317773
GTATTCG2101.648398E-523.9880451
GTATTAC6050.023.7897991
TTGCTCT35900.023.630434
Produced by FastQC (version 0.11.5)