FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348361

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348361
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494671
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7450.039.621968
GTCTCGC8650.035.2344251
CTGTCGC10100.031.3645949
TCGCTCT10850.029.8600944
GTCTTGC18500.028.2419571
CTCGCTC10800.026.6651863
GTCTTAC7350.026.6569481
TCTCGCT11400.026.5248432
GTATAAG7600.024.825261
CTCGCGT1500.0015568723.9986679
CGCTCTG12350.023.9015065
GTCTCAC20400.023.1215651
GGAGTGC48850.022.6967955
TAGCGCT2603.4074947E-622.1526174
GTCTAGC9400.021.6154461
GTATAAA9750.021.5837751
GAGTGCA55700.021.3273626
GTACAGA11700.021.0875971
GGCTGGA69350.020.927511
GTCTTAG7750.020.5994191
Produced by FastQC (version 0.11.5)