FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348362

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348362
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2494671
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10450.034.4366951
GTCTTGC19800.032.714861
TACCCGT2051.2376404E-831.56235
TCTGTCG10200.031.0121968
TCGCTCT12050.029.8306664
CGCTCTG11150.027.725135
CTCGCTC12350.026.1954333
CTGTCGC13100.025.793289
TTGCTCT25800.025.0785734
CTTGCTC25400.024.3413563
GTGTTAG15250.023.5976031
GTCCCGG4700.022.9700091
GTGTTAC7900.022.7761671
ATACCGC1902.323227E-422.7027076
GTACTAG4800.022.4914671
GGAGTGC51250.022.163755
TCTCGCT14300.022.1263542
ATACCCG1952.7696582E-422.1205884
CTTCGTA2303.3556516E-521.8801469
GAGTGCA56400.021.6695486
Produced by FastQC (version 0.11.5)