FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7430921
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG35600.026.8966718
GTATTAA16400.024.1781981
GTATTAG17550.023.8262651
GTCTCGC40250.023.6435761
GTGTTAG49100.023.640111
GTATAGA16850.023.532491
GTATAAC10200.022.6180361
GTCTTGC78650.022.4580731
GTCTTAC27100.022.3467881
GTATAAT14300.022.1830751
TCGCTCT49000.020.1295034
GTGCTAC27550.019.6265851
GTATATA18750.019.609841
GTTAGCC52000.018.835183
GTGTAAC19500.018.4858951
GTATAGG14500.018.3966521
GTGTAAG34400.018.2333281
TGTTAGC55050.018.1845842
GTGTTAC27350.017.9248981
GTTTCAC132600.017.8684393
Produced by FastQC (version 0.11.5)