FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2754922
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10850.049.1028988
GTCTCGC12200.039.271241
CTGTCGC14650.038.3320549
GTCTTGC25550.033.241871
TCGCTCT13900.032.114254
TCTCGCT14900.029.4757232
CGCTCTG13700.029.4298695
GTCTTAC9300.028.8808061
GTATTAG9650.027.8333131
CTCGCTC15100.027.6548883
GTGTTAC10650.025.9014781
TTGCTCT32900.025.6041774
GGTATAC5500.025.077341
GTGTTAG19850.024.5021841
GTCTCAC31050.023.379161
TTACACT12500.023.0393854
CTTGCTC33900.022.7250563
GTATTAA8350.022.603591
GTTTCAC41850.022.3649943
GTGTAGG16300.022.2675761
Produced by FastQC (version 0.11.5)