FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2754922
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11500.044.3861778
GTCTTGC23950.038.1616441
TCGCTCT12550.037.8076444
GTCTCGC12650.037.5476071
CTGTCGC15650.032.6160439
CTCGCTC13700.029.9111883
TCTCGCT15650.029.8673462
TTGCTCT32600.029.7711454
CGCTCTG13650.028.4407145
GTATTAG8150.028.2566871
TTCACCG8700.025.616625
ATACCGA2001.1383414E-525.1621326
GTCTCAC30750.024.8078781
GTATTAT7350.024.4783151
GTGTTAG18350.023.9233881
TCTTGCT39550.023.818942
GAGTGCA67400.023.7861616
CCTAGCG3052.2280801E-823.571519
GTCCTAT7400.023.3404061
CTTGCTC34650.023.2377533
Produced by FastQC (version 0.11.5)