FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6515518
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC24200.021.5131821
GTCTCGC35800.020.6871191
TCTGTCG33500.019.9853068
GTATATA14400.019.8592931
GTGTTAG39450.019.7024631
GTATAGA15900.019.3692971
GTATAGT12650.019.1286851
GTATTAT15350.019.1079141
GTCTTGC62550.018.6393811
TATACTG14800.018.484655
GTATAAG12300.018.4806941
GTATAAC7950.018.4469491
TCGCTCT45150.018.1775934
GGTATAC10500.018.1570681
TAATACT13150.018.0666454
GTACTAT11950.017.7947441
GTATTAG13650.017.7273161
GTCTAGC19800.017.0354791
GTACAGA36250.016.5869871
GTTAGCC40500.016.5316893
Produced by FastQC (version 0.11.5)