FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348372

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348372
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2593330
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10150.038.956718
GTCTTGC22600.035.6716421
TCGCTCT10900.033.6379364
GTCTCGC11700.031.3760171
TTACACT11550.028.0101974
GTATTAA6750.027.7257351
CGTATAT1050.00798616127.4210551
GTATAAC3505.456968E-1226.7355291
GTGTTAG15750.026.5070191
ACCGTGT7900.026.391048
CTGTCGC14700.025.9205479
GGTATAC5000.025.9128971
GTGTAAA16350.025.5342851
CGCTCTG11900.025.3739435
TCTCGCT14200.025.3212172
GTATTAC5450.025.094041
CTCGCTC13600.024.845323
GTGTAAT10900.024.433671
GTGTTAT10550.023.8797111
TACACTG14300.023.6291165
Produced by FastQC (version 0.11.5)