FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383501
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9850.043.484631
TCTGTCG9150.040.1287358
TCGCTCT11450.037.7293624
CTGTCGC12050.032.263629
CTCGCTC12850.031.937833
TCTCGCT13250.030.9736712
GTCTTGC20150.028.822721
ATACTGA9200.028.1739926
TTCACCG7700.028.0520235
CGCTCTG13150.027.9240325
GTATTAT7400.026.4881731
TCACCGT8000.026.100076
GTGTTAG16700.026.082831
ACCGTGT8300.026.022538
GTATTAG8000.025.4090251
GTCCAGA15500.025.2919331
GCGTAGT2901.17561285E-825.0335251
GTGTTAT8450.024.9150221
GTTTCAC33550.024.2504393
GTTAGCC13600.023.8235973
Produced by FastQC (version 0.11.5)