FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2383501
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10100.054.8605541
TCTGTCG11000.049.670858
TCGCTCT12600.045.645734
CTCGCTC13400.040.774583
TCTCGCT13050.039.672052
GTCTTGC19800.039.6143151
CTGTCGC13900.039.3078659
CGCTCTG13700.038.8321845
AGTACCG1602.0061561E-631.4527595
GTCTTAC8750.030.4287781
GTACGAA1204.241481E-429.983311
TTGCTCT28200.028.0429864
GTGTAAC6550.027.465631
GTCTCAC25550.027.3194331
TCTGTCA28450.027.0384758
ACCGTGT6600.026.1425518
GTTTCAC31350.025.913233
GTGTTAG16400.025.449251
CTTGCTC29100.025.1992653
GTATAAT8450.024.6963141
Produced by FastQC (version 0.11.5)