FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172854
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8250.045.379468
GTCTTGC19900.034.6234361
CTGTCGC11650.033.371669
TCGCTCT8500.033.0335774
GTCTCGC8800.032.966811
GTGTTAC9250.030.5789431
CTCGCTC9500.028.7985043
GGTATAC4150.027.962211
GTCTTAC6650.027.2657811
TCTCGCT10150.026.9542642
GTATAAT7050.025.7187881
GTGTAAA11900.025.597761
GTTGCGC1701.0292289E-425.597761
CGCTCTG10550.024.5674445
GTACTAT4850.023.9264281
CTTCGTA2452.0334282E-623.5089829
GTATTAT5900.023.3561461
TTGCTCT27350.023.1651574
CCAACGG2202.3538749E-522.90793
GTGTAGC8900.022.8174781
Produced by FastQC (version 0.11.5)