FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739046
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11250.043.5175258
GTCTCGC12050.033.7227751
CTGTCGC14950.033.228889
TCGCTCT13350.031.819584
CGCTCTG13800.027.6516135
TCTCGCT14650.027.0301672
CTCGCTC14900.026.0934313
GTCTTGC22500.025.478091
GTGTAAC5750.025.2397171
GTGTATA9450.024.5719991
GTGTTAG16250.024.5601881
GTGTTAA9250.024.3188081
GTCTCAC27700.023.8387761
GGCGTAC1550.001799994423.4078031
GTCTTAC8800.023.0886041
GTTAGCC13250.022.8221783
GTACGGT2353.91633E-521.4463733
GAGTGCA65900.021.195326
GTACTAG4501.3096724E-1020.9629881
GTATTAA6600.020.8896881
Produced by FastQC (version 0.11.5)