FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2739046
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10900.038.2557038
GTCTTGC22050.033.292491
GTCTTAC7950.031.6851431
TCGCTCT11900.029.6035484
CTGTCGC15850.027.6691119
GTCTCGC12100.027.3606971
TCTATCG1358.492376E-426.6275318
GTCTAGC9650.026.1033021
GTCTCAC27900.024.2481371
CGCTCTG13600.023.7885655
GTATTAA5900.023.1769521
GTCTTAG8700.023.162931
CTCGCTC13750.023.0061863
GGAGTGC64850.022.7268125
GAGTGCA66250.022.6806286
CTAGTAC4455.456968E-1222.618443
GTGTTAG15450.022.3597761
TACCCGT1952.7691232E-422.1213345
CTGGAGT73250.022.0835843
GTGCTAA8900.021.8337571
Produced by FastQC (version 0.11.5)