FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348386

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348386
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2329616
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10500.046.558918
GTCTCGC11350.039.9582251
GTCTTGC19600.039.2996981
TCGCTCT11650.038.8774534
CTGTCGC14550.033.1051069
CGCTCTG13600.031.1886335
CTCGCTC14400.030.9536913
GTATTAA6050.029.7472061
TCTCGCT14800.029.1543432
TTGCTCT26300.027.3355274
GTGTTAG12400.026.7053131
GTACTAT4600.026.6043471
GGTATAC4350.026.4784321
TCCGTTG2456.994014E-826.4094688
GTTTCAC33150.025.156933
GTATATA7750.025.0797731
GGGTTTC34800.024.6166671
CTTGCTC27800.024.567563
TATACTG6150.024.5486355
TCTGTCA27150.024.0965448
Produced by FastQC (version 0.11.5)