FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348397

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348397
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324914
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC5000.038.908021
CTGTCGC4800.037.4987039
GTCTCGC5250.037.055261
TCTGTCG5200.035.9987568
GTCTTGC12600.035.4541051
GTACGAA850.002826934733.9067731
TACACCG950.004891430530.3147415
TTCACCG3600.029.9989625
TCGCTCT5350.029.6064534
TCGATCT5300.028.5273139
TAGTACT3052.5465852E-1128.3268894
TGTTCCG1555.768724E-527.875
GTTCCGC1050.00794612527.448341
GTATAAG3950.027.3614791
TCTCGAT4800.026.9990677
CTCGATC4850.026.7207268
GTACGGA1100.00996943326.2006871
GTATTAT4150.026.0428541
GTGTTAG8100.025.7963541
GGGTTTC20550.025.5950761
Produced by FastQC (version 0.11.5)