FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348398

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348398
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324914
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAA700.001092912941.1462971
GTCTCGC5200.040.1572041
CGTTTCG907.794925E-540.0003289
TCTGTCG6600.039.2730458
GTATAAG3700.038.9221731
GTCTTGC11350.036.796031
GTGTAAC4850.035.6318441
GTATTAA3150.034.288581
TGATCGA850.002836823733.882639
GTCTTAC4500.030.402541
TTGCTCT15550.030.0955724
TCGCTCT6900.029.2165264
GTGTTAG8150.029.1558131
GTATTAG3500.028.8024081
CGTACTG1000.006283802428.8002345
GTAACCG1000.00628496128.7991493
GTAATAC3103.092282E-1127.8701423
GTATAAA5450.027.7454381
GTACTGG6300.027.4308661
GGGTTTC19950.027.4308641
Produced by FastQC (version 0.11.5)