FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1142184
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5200.060.921898
GTCTCGC5650.051.014891
CTGTCGC6550.050.56399
TCGCTCT5850.045.5375754
GTATAAT3200.045.0365831
TCTCGCT6850.041.0260282
GTATTAG2900.037.2716561
CTCGCTC6950.035.251663
GTCTTGC11200.034.742511
CTACGCT1251.326836E-534.5593264
GTCTTAC4000.034.2278061
TAATACT2809.094947E-1230.8565444
CGCTCTG7500.030.71945
GTATATA3550.030.4472681
GTGTAAT4050.030.2467921
GTATAGG2502.6120688E-928.8234141
TATAATA2354.561298E-827.5968882
GTGTAAC3153.6379788E-1127.450871
TGCTACG1050.007942127.450872
TTGCTCT14050.026.1347244
Produced by FastQC (version 0.11.5)