FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348410

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348410
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333253
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG701.7672239E-551.4284365
GTCTCGC5900.051.259871
TCGCTCT6350.045.3542144
GTCTTGC11950.039.770061
TCTGTCG6850.037.8393178
CTCGCTC8300.033.8312383
CGCTCTG8250.033.1635485
TCTCGCT8500.033.035212
TTGCTCT17300.029.1328724
GTCTAAC3002.0008883E-1128.8031651
CCGTTGA1255.3678843E-428.7999259
GTGTAAT4850.028.2092861
TCTTGCT17900.027.7541182
CTTGCTC17850.027.0251393
GTCTCAC14850.026.1846961
GTCTTAC4950.026.1846961
GTATAAT3659.094947E-1225.6466541
TTACACT5350.025.5700264
GGAGTGC29600.025.2972325
GAGTGCA32550.025.2165266
Produced by FastQC (version 0.11.5)