FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348417

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348417
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441819
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6550.057.158978
GTCGTAC605.097991E-448.0371931
GTCTCGC7850.047.731221
CTGTCGC8900.045.302319
TCGCTCT8300.044.2399334
GTCTTGC12650.041.58161
CTCGCTC9100.040.3829153
TCTCGCT9150.040.1622472
GGTATAC2700.037.3622631
TAACGTC1102.5380502E-432.7265054
CGCTCTG9950.032.5620545
GTCTCAC17000.029.670031
TTGCTCT15650.029.4434034
GTGTTAC5300.028.5504071
TCACTCT17150.028.5474364
GTGTAAC4200.027.4498251
TTCACCG4200.027.427935
TCCCGAT1607.160778E-526.999377
GTGTAAT5650.026.7817971
GTATAAG3555.456968E-1226.3866271
Produced by FastQC (version 0.11.5)