FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348418

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348418
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441819
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC6850.038.8915181
GTCTTGC12950.034.471941
GTCTTAC4650.030.9685461
TCTCGCT8450.030.674292
CGCTCTG7650.029.1762165
TCTGTCG9150.029.1145028
GTGTTAG8750.027.1549911
GTCTCAC17850.027.0259131
GTTAGCC8100.026.6664353
GTGTAAG6100.025.967891
GTATTAC2508.376992E-825.9206751
CTCGCTC8650.025.8032443
TATACTG3109.313226E-1025.5481665
TCGCTCT8250.025.3088724
GTCTACG1450.001277005424.8282321
AACGTCC1450.001277263524.827377
TACTGCG1450.001277263524.827377
GTTTCAC19450.024.8018423
GTGTTAC5850.024.6160241
GGGTTTC20800.024.5775621
Produced by FastQC (version 0.11.5)