FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348423

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348423
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297069
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC5350.047.148991
TCCGTAC1002.8488448E-643.242363
TCGCTCT5850.040.614464
TCTCGCT6300.036.607292
ATCGATT800.002107443335.999188
TCTGTCG4700.035.2332428
CTCGCTC6800.032.8557133
GGGTTAA3550.032.482521
GTATTGC3050.030.7186151
GTACGAA950.004867181630.3455141
CTGTCGC6150.028.0969229
CGCTCTG7200.027.9993635
TATACTG3403.6379788E-1227.5287885
GTTTCAC18250.026.8537033
GTGTAAC3505.456968E-1226.7690771
GGTATAC2757.210474E-926.2074891
GTCTTGC11050.026.0889031
GTGTAGA5550.025.9713841
ATACTGA4450.025.8870536
GTAACCC3751.2732926E-1124.9844743
Produced by FastQC (version 0.11.5)