FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005348424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005348424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1297069
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC6500.037.670121
GTGTTAG8350.031.911461
GCGTATA1402.875813E-530.8641741
TCTCGCT7250.030.7860982
TAATAGT3350.030.0894474
GTCTTGC10650.029.7532561
CTCGCTC6800.029.6469553
GTTTCAC17700.029.2880343
GTATTAC2502.6302587E-928.8065621
TCGCTCT7100.028.3942684
GTATTCG1050.00796518827.434821
GTATAAT3153.8198777E-1127.434821
TATCCCG1358.4176194E-426.6665735
GTATAAG3607.2759576E-1226.0059261
GTGTAAT4750.025.7742921
TCTGTCG7300.025.6437478
GGGTTTC19400.025.6140821
CGCTCTG7650.025.4116765
GTATAGA3701.0913936E-1125.3030641
TACACTG7550.024.7946175
Produced by FastQC (version 0.11.5)